Restriction sites are marked: BamHI (B), HindIII (H), PstI (P), EcoRI (RI), SmaI (Sm), SstI (Ss), XbaI (X), XmaI (Xm).

This vector is described in Thummel et al. (1988) Gene 74: 445-456. It requires both a promotor and in-frame AUG codon for lacZ expression in transformed flies.